Tissue Culture Experiment of Eucalyptus deglupta

In 2023,a tissue culture technology system study was conducted at the Fujian Forestry Science and Technology Test Center using stem segments of Eucalyptus deglupta with semiwoody bud points as explants.The optimal conditions for sterilization of explants,induction of adventitious buds,subculture proliferation,and rooting induction were screened.The results showed that after disinfecting with 75% alcohol for 8 seconds,disinfecting with 0.1% mercuric chloride for 8 minutes can obtain more sterile explants,which is the best sterilization treatment method;After 20 days of cultivation in improved MS+6-BA 0.2 mg·L^-1+NAA 0.1 mg·L^-1+sucrose 30 g·L^-1,the induction rate of Eucalyptus deglupta reached 86.0%,making it the optimal induction medium;after 20 days of cultivation in an improved MS+6-BA 0.25 mg·L^-1+IBA 0.05 mg·L^-1+NAA 0.10 mg·L^-1+sucrose 30 g·L^-1 medium,the average proliferation coefficient reached 2.23,making it the optimal subculture medium;After 30 days of cultivation in improved MS+IBA 0.30 mg·L^-1+NAA 0.05 mg·L^-1+sucrose 20 g·L^-1+activated carbon 0.1 g·L^-1,the average rooting rate reached 94.0%,with 3~6 roots and 1~2 cm root length,making it the optimal rooting culture.The research results can provide technical support for establishing a tissue culture technology system for Eucalyptus deglupta and for large-scale seedling cultivation.