Abstract: In order to explore the effects of soil total pphosphorus and available phosphorus on different fungal functional groups, soil samples were collected from 25 hm2 of subtropical forest dynamic plot in Baishanzu National Nature Reserve, Zhejiang Province.The composition of Soil fungal community was determined by Illumina high-throughput sequencing technology, and the effects of soil total phosphorus and available phosphorus on arbuscular mycorrhizal fungi, ectomycorrhizal fungi, plant pathogens and soil saprotrophytic fungi were analyzed combined with soil physical and chemical properties.Furthermore, the co-occurrence networks of soil total phosphorus and available phosphorus with fungal functional groups was constructed. The results showed that soil total phosphorus and available phosphorus significantly increased the richness of ectomycorrhizal fungi and plant pathogens, and soil total phosphorus could significantly increase the richness of soil saprotrophtic fungi, while available phosphorus reduced the richness of arbuscular mycorrhizal fungi. In addition, soil total phosphorus and available phosphorus can indirectly drive the change of fungal community structure by affecting different fungal functional groups and significaltle predict the community of functional groups. In summary, soil total phosphorus and available phosphorus can drive the community changes of different fungal functional groups.