Abstract: In order to improve the transplant survival rate of tissue cultured seedlings of Bougainvillea.This study used the root tissue culture seedlings of Bougainvillea 'Zhongmin No.1' as the experimental material,orthogonal experimental design,comparative experiments were carried out on influencing factors such as seedling refining time,fungicide concentration,substrate and rooting solution concentration,in order to screen out the best conditions for the transplantation of tissue cultured seedlings of Bougainvillea.The results showed that different seedling mixing time,fungicide concentration,substrate and rooting solution concentration had significant effects on the transplanting survival rate and new leaf rate of Bougainvillea seedlings.Among them,A₂B₂C₂D₂ is the best treatment combination for tissue culture seedling transplanting of 'Zhongmin No.1' Bougainvillea,that is,before transplanting tissue cultured seedlings of Bougainvillea,the seedlings were harvested for 10 days with high light closed flask,clean the medium after leaving the bottle,sterilize it with 1500 times the liquid of iomediuron,ABT root powder dipped in 1000 times liquid was planted in peat soil:coir =4:1 cultivation substrate.The best combination of transplanting conditions selected is of great significance for guiding the industrialized seedling raising and transplanting tending management of tissue cultured seedlings of Bougainvillea.