Abstract: In 2021,in Changkou Village,Jianle County,Sanming City,Fujian Province,three types of forest stands (Pinus massoniana Lamb-Russula symbiotic system,referred to as YXA;mixed Castanopsis carlesii and Pinus massoniana Lamb forest-Russula symbiotic system,referred to as YXB;mixed Pinus massoniana Lamb and Cunninghamia lanceolata forest-Russula symbiotic system,referred to as YXC) were studied.The rhizosphere soil of Russula was subjected to metagenomic sequencing and analysis to investigate the influence of different types of forest stands on the microbial community changes in Russula soil.The results showed that there were differences in microbial diversity in the rhizosphere soil of Russula under the three types of forest stands,with the diversity ranking as YXB>YXC>YXA.YXB has richer microbial diversity in rhizosphere soil and higher diversity index of rhizosphere fungi community.The dominant microbial flora in rhizosphere soil are Proteobacteria,Actinobacteria and Acidobacteria.The function prediction of KEGG 3 pathway shows that YXA group mainly focuses on protein kinase pathway,transcription mechanism pathway,replication and repair pathway and transcription factor pathway.In YXB group,there were six pathways with high abundance: chaperone and folding catalyst,peptidase and inhibitor pathway,glycosyltransferase pathway,glutathione metabolism,cell cycle blindness and lipopolysaccharide biosynthesis protein.There were three pathways with high abundance in YXC group: porphyrin and chlorophyll metabolism,fructose and mannose metabolism and amphetamine acid metabolism.Russula will specifically select soil microorganisms that were beneficial to its growth according to its growth needs,and form different microbial communities.Therefore,it was suggested to popularize YXB (interplanting Russula in mixed forest of Castanopsis carlesii and Pinus massoniana) model,which can provide theoretical basis for artificial propagation of Russula,which is of great significance for sustainable utilization of Russula,expanding the scale of edible fungi under forests and improving economic benefits.