贝壳杉种子耐盐性及耐盐机理探究

Study on Salt Tolerance and Mechanism of Seed of Agathis dammara (Lamb.) Rich.& A.Rich.

  • 摘要: 为明确贝壳杉种子的耐盐性及耐盐机理,于2022年在厦门市园林植物园实验室开展盐胁迫下贝壳杉种子的萌发试验,设置0%(对照)、0.3%、0.6%、0.9%、1.2%、1.5%、1.8%、2.4%、3%共9个NaCl质量分数梯度,测定不同盐浓度处理下种子的发芽率、发芽指数、活力指数、盐害指数、胚长度,胚及胚乳中K+、Na+、Cl-含量,以及丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性等生理指标。结果表明:贝壳杉种子萌发期的耐盐阈值为1.27%,高盐胁迫对贝壳杉种子的萌发与胚生长有抑制作用,NaCl质量分数≤1.5%时,生理干旱的危害大于离子毒害,当及时补充淡水后可部分恢复萌发。贝壳杉种子在盐胁迫下萌发时,胚乳吸收环境中的盐离子后脱离幼苗,可降低幼苗生长环境的盐浓度;萌发时种子能积极向胚输送K+,以利胚维持较高的K+/Na+,且胚中的SOD与POD表现出协同作用,减轻了种子的盐胁迫危害。综上,贝壳杉种子具有较强的耐盐性,可在我国华南滨海地区土壤含盐量不超过1.27%的盐碱地上育苗及应用。

     

    Abstract: To study the salt tolerance and salt tolerance mechanism of the seed of Agathis dammara,the germination experiment under salt stress was carried out in the laboratory of Xiamen Botanical Garden in 2022,and 9 NaCl mass fractions (0,0.3%,0.6%,0.9%,1.2%,1.5%,1.8%,2.4%,3%) were set up.The germination rate,germination index,vitality index,germ length,and the concentration of K+,Na+,Cl-,MDA,SOD and POD were measured.The results showed that the threshold of salt tolerance was 1.27%,and the high salt concentration would inhibit seed germination and embryo growth.When the NaCl mass fraction was less than 1.5%,the harm of physiological drought was greater than that of ion toxicity,and the germination can be partially restored if fresh water was added in time.During germination,the endosperm absorbs salt ions from the environment,finally leaving the seedlings to reduce the salt concentration of the growth environment.At the same time,the seeds of A.dammara actively transport K+ to the embryo to help it maintain a high ratio of K+ to Na+,combined with the co-action of SOD and POD,to reduce the salt damage to the seed.In conclusion,the seed of A.dammara has a strong salt tolerance and can be sown on the saline-alkali soil in the coastal area of South China,where the soil salt content does not exceed 1.27%.

     

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