Abstract: In order to clarify the occurrence of Prunus necrotic ring spot virus (PNRSV) in Rosaceae plants in Yangzhou City,Jiangsu Province,the sequences of the coding region of the coat protein (CP) of four isolates were determined by ELISA on 243 leaf samples of Rosaceae plant collected from different spots in Yangzhou City,which were combined with the sequences of related sequences reported in GenBank for diversity and phylogenetic analyses. The results showed that the detection rate of PNRSV on Rosaceae plant in Yangzhou City,Jiangsu Province,China was 13%. Sequence analysis showed that the nucleotide sequences of the PNRSV isolates obtained in this study were in agreement with those of the other 286 isolates in GenBank at a rate ranging from 82% to 99%,and the phylogenetic analysis showed that the PNRSVs could be classified into four groups,with clearer geographic and host specificity among the groups. The PNRSV isolates obtained in this study were all located in the PV32 group. In conclusion,PNRSV has already occurred and become prevalent in Yangzhou City and should be taken seriously.