福建省杉木赤枯病病原菌鉴定及防治药剂筛选

Identification of the Pathogens Causing Cunninghamia lanceolata Copper Blight in Fujian Province and Screening of Fungicides

  • 摘要: 为明确福建不同地区杉木赤枯病菌的种类及有效防治药剂,从福建东、南、西、北4个地区采集杉木赤枯病样本并进行分离,结合形态学特征与分子生物学技术,对病原菌株进行鉴定;同时,采用菌丝生长速率法评估多菌灵、苯醚甲环唑及异菌脲对杉木赤枯病菌的抑制作用。结果表明,共采集到4种拟盘多毛孢属(Pestalotiopsis)真菌,包括小孢拟盘多毛孢(Pestalotiopsis microspora)、茶拟盘多毛孢(Pestalotiopsis theae)、木防己拟盘多毛孢(Pestalotiopsis cocculi)和欧克斯安拟盘多毛孢(Pestalotiopsis oxyanthi)。其中,小孢拟盘多毛孢和茶拟盘多毛孢致病性强,分布区域最广。杀菌剂毒力测定表明,多菌灵抑制拟盘多毛孢属真菌效果最佳,苯醚甲环唑次之,异菌脲最差,相应的EC50平均值分别为0.19、10.42、20.99μg·mL-1。结果表明,福建地区杉木赤枯病菌以小孢拟盘多毛孢和茶拟盘多毛孢为主,多菌灵防治杉木赤枯病可能有较好的应用前景。

     

    Abstract: To identify the copper blight pathogen of Chinese fir in Fujian Province and identify efficient fungicides, we collected samples of copper blight from four regions in eastern, southern, western and northern Fujian.The samples were then isolated, and the pathogenic strains were identified based on morphological characteristics and molecular biology techniques.Additionally, the inhibitory effects of carbendazim, tebuconazole, and flusilazole on the red rot pathogen were assessed using the mycelial growth rate method.And the inhibitory effects of three commonly used fungicides on copper blight pathogen were determined using the mycelial growth rate method.The results showed that four species of Pestalotiopsis were collected, including Pestalotiopsis microspora,Pestalotiopsis theae,Pestalotiopsis cocculi and Pestalotiopsis oxyanthi.Among them, P.microspora and P.theae were highly pathogenic and had the widest distribution areas.Toxicity tests of fungicides indicated that carbendazim was the most effective in inhibiting the mycelial growth of Pestalotiopsis species, followed by difenoconazole and iprodione, with EC50 values of 0.19 μg·mL-1,10.42 μg·mL-1 and 20.99 μg·mL-1.The study showed that P.microspora and P.theae were the primary pathogens responsible for copper blight in Fujian area, and that carbendazim might have a better application prospect in the control of copper blight.

     

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