一株虫生真菌的鉴定及其对杉肤小蠹的致病力

Identification of Entomopathogenous Fungus and Pathogenicity Test of Phloeosinus sinensis Adult

  • 摘要: 于2023年从漳浦县1头自然罹病死亡的天牛幼虫僵虫中分离并纯化得到1株虫生真菌BFZ-103。通过形态学和分子系统发育分析相结合的方法鉴定该虫生真菌,观察并记录虫生真菌生物学特性及测定其对杉肤小蠹(Phloeosinus sinensis)成虫的致病性。结果表明:菌株BFZ-103为布氏白僵菌(Beauveria brongniartii),在25℃条件下,用CDA培养的菌株生长速率较高,培养15 d后菌落直径为(72.36±0.67)mm;而SDAY培养的菌株产孢量最高,为(9.63±1.24)×106个·cm-2。菌株BFZ-103对杉肤小蠹成虫致病力较高,接种15 d后死亡率达80.9%。综上,布氏白僵菌菌株BFZ-103对杉肤小蠹的生物防治具有较高的开发潜力。

     

    Abstract: In 2023,a strain of entomopathogenic fungus BFZ-103 was isolated from the infected larva of Cerambycidae sp.in Zhangpu County.To clarify the taxonomic status of strain BFZ-103,by combining morphological and molecular biological evidence to identify the entomopathogenic fungus.We observed and recorded the entomopathogenic fungus biological characteristics and determined its pathogenicity to adult Phloeosinus sinensis.The results indicate that strain BFZ-103 was identified as Beauveria brongniartii.The strain cultured with CDA had a higher growth rate at 25 ℃,after 15 days of cultivation, the colony diameter was(72.36 ± 0.67)mm.The spore production of strain BFZ-103 was(9.63±1.24)×106 spore·cm-2 15 days after culture on SDAY at 25 ℃.The Beauveria brongniartii strain BFZ-103 showed high virulence to the adult of Phloeosinus sinensis.The mortality of the adult of M.alternatus reached 80.9% 15 days after inoculation.In summary, the strain BFZ-103 has the good potential to be developed as biocontrol agents for Phloeosinus sinensis.

     

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