Abstract: To accurately quantify morphological variations of entire,three-lobed,and five-lobed leaves in Ficus hirta,the phenotypes of 602 leaves from 131 individuals collected in 14 prefecture-level cities of Guangxi were studied using Geometric Morphometrics (GMM)methods. Thirteen landmarks were labeled using Image J,and then Generalized Procrustes Analysis(GPA),Principal Component Analysis(PCA),and K-means clustering were performed using Morpho J software. The results showed that the cumulative contribution rate of the first two principal components in the symmetric group was 57.122%,and that of the asymmetric group was 62.273%. The number of leaf lobes and the depth of lobes were the main driving factors of morphological variation. K-means clustering analysis revealed that the entire leaves,the three-lobed leaves,and the five-lobed leaves were significantly different in overall shape and local features,and only 5.8% samples exhibiting morphological transition. The quantitative analysis confirmed that the number of lobes was the core index of morphological differentiation of the leaves in Ficus hirta. The morphological differences between the entire leaves and the lobed leaves might correspond to different ecological adaptation strategies,in which the former might adapt to the arid environment by reducing the surface area,while the latter might improve the efficiency of light energy utilization by increasing the photosynthetic area.