Abstract: This study investigated the effects of three cultivation measures(cultivation medium,water regimes and shading)on the accumulation of nutrient elements and bioactive compounds in leaves of Cyclocarya paliurus seedlings. The cultivation media included four treatments:peat soil and perlite(10 ∶ 1,J1);yellow soil,plant ash,manure and perlite(5 ∶ 2 ∶ 1 ∶ 2,J2);yellow soil(J3);peat soil and yellow soil(10 ∶ 1,J4);water treatments were set as W1,W2,W3 and W4,representing 10% ~ 20%,30% ~ 40%,50% ~ 60% and 70% ~ 80% of field capacity,and shading treatments were set as SH0,SH1 and SH2,representing 60% ~ 70%,30% ~ 40% and 10% ~ 20% of full sunlight. The results showed different treatments had significant effects on nutrient and bioactive compound accumulation. Among nutrient elements,molybdenum(Mo) showed the highest content(10~ 40 mg·kg^-1),followed by boron(B),iron(Fe) and zinc(Zn)(48~ 600 μg·kg^-1),vanadium(V),chromium(Cr) and nickel(Ni) were the lowest(0. 5 ~ 5 μg·kg^-1). J3 significantly affected the content of B and Ni,Mo content in J1 initially decreased and then increased with the increasing water capacity in medium,while the contents of Mo,Fe,Ni,Cr and V reduced,Zn content increased and then decreased,but B decreased and then increased with the increasing shading nets. For bioactive compounds,total triterpenes were the most(150 ~ 400 mg·g^-1),followed by polysaccharides and total flavonoids(50~ 250 mg·g^-1),total polyphenols was the lowest(20 ~ 100 mg·g^-1). J3 significantly increased total flavonoids and polyphenols,W4 promoted the content of total flavonoids,polyphenols and triterpenes,while SH1 only elevated the content of total triterpenes. Membership function and correlation analyses revealed treatment effects followed in order of SH>W>J,and Ni positively correlated with Fe,B and Zn,Mo also had positive relationship with Cr and V,and positive relationship existed between total flavonoids and polyphenols,while total polyphenols negatively correlated with B. Shading treatment significantly affected nutrient and bioactive compound accumulation,and a relationship existed between nutrient and bioactive compound. Based on its related biomass characteristics,optimal cultivation in J1 medium with 50% ~ 60% water capacity in medium and two-layer shading effectively promoted nutrient and bioactive compound accumulation. It provides a theoretical basis for the cultivation and production of Cyclocarya paliurus and enhances its comprehensive utilization value.