普通油茶杂交F1代优良无性系的遗传测定及选择

Genetic Testing and Selection of Superior Clones in F1 Hybrid Generationof Camellia oleifera

  • 摘要: 为筛选油茶(Camellia oleifera)优良无性系,对17个普通油茶杂交F1代无性系进行多点联合测定试验。结果表明:17个普通油茶无性系间果实性状存在显著或极显著差异,并且单果质量、单株产量、每平方米冠幅等产量性状无性系×地点交互效应等均存在显著差异。多点联合测定的果实性状重复力值在0.589 ~ 0.862之间,而单一地点测定的果实性状重复力普遍低于多点联合测定值。通过沙县、福安、福州、仙游4个试验点的多点联合测定,筛选出ZY3、ZY10、ZY20、ZY22、ZY24、ZY30等6个杂交F1代优良无性系,其鲜出籽率、干出籽率、出仁率、种仁含油率、每平方米冠幅产果量范围分别为40.66%~45.47%、25.41%~26.68%、59.71%~65.05%、45.05%~46.58%、1.67~2.03 kg·m-2,6 a平均产油712.73~903.19 kg·hm-2,比对照增产37.21%~73.87%,比17个参试无性系的平均产油量高15.82%~46.77%,达到油茶优良无性系选育标准。6个F1代优良无性系茶油品质优异,油酸、不饱和脂肪酸、角鲨烯、β-谷甾醇、α-维生素E、总多酚含量范围分别为78.78%~81.46%、89.15%~90.05%、93.95~257.90 mg·kg-1、195.80~246.23 mg·kg-1、83.76~149.00 mg·kg-1、8.18~24.36 mg·kg-1。相关分析表明,果实性状与茶油脂肪酸组成及生物活性物质间存在一定的相关性,其中油酸、不饱和脂肪酸、α-维生素E含量与单果质量呈显著正相关,角鲨烯含量与单株产量和年均产油量分别呈极显著和显著负相关,总多酚含量分别与果径、种仁含油率呈显著相关。

     

    Abstract: In order to screen excellent clones of Camellia oleifera,a multi-site joint determination experiment was carried out on 17 hybrid F1 clones of C.oleifera.The results showed that there were significant or extremely significant differences in fruit traits among the tested C.oleifera clones,and the clone × location interaction effects of single fruit mass,yield per plant,and yield per square meter of crown width were significant.The repeatability of each fruit trait in the multi-site joint determination was relatively high,with repeatability values ranging from 0.589 to 0.862.However,the repeatability of most fruit traits at individual locations was lower than that observed in the multi-site joint determination.Compared with the control,6 hybrid F1 clones such as ZY3,ZY10,ZY20,ZY22,ZY24 and ZY30 were screened out at the experimental sites of Shaxian,Fu'an,Fuzhou and Xianyou.The ranges of fresh seed extraction rate,dry seed extraction rate,kernel extraction rate,oil content in kernel,and fruit yield per square meter of crown width were 40.66%~45.47%,25.41%~26.68%,59.71%~65.05%,45.05%~46.58%,1.67~2.03 kg·m-2,respectively.The average annual oil production in 6 years was 712.73~903.19 kg·hm-2,which was 37.21%~73.87% higher than that of the control and 15.82%~46.77% higher than the average oil production of the 17 tested clones,meeting the selection criteria for excellent clones of C.oleifera.The tea oil quality of these 6 excellent F1 clones was excellent.The ranges of oleic acid,unsaturated fatty acids,squalene,β-sitosterol,α-tocopherol and total polyphenol contents were 78.78%~81.46%,89.15%~90.05%,93.95~257.90 mg·kg-1,195.80~246.23 mg·kg-1,83.76~149.0 mg·kg-1,8.18~24.36 mg·kg-1,respectively.Correlation analysis showed that there was a certain correlation between fruit traits and the fatty acid composition and bioactive substances of tea oil.Among them,the contents of oleic acid,unsaturated fatty acids,and α-tocopherol were significantly positively correlated with single fruit mass,squalene was extremely significantly and significantly negatively correlated with yield per plant and annual averaye oil preduction,respectively,and total polyphenol was significantly correlated with fruit diameter and oil content in kernel,respectively.

     

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