栀子‘灵地珍栀’水培扦插植物生长调节剂优化试验

Optimization Study on Growth Regulators for Water-Cultured Cutting Propagation of Gardenia jasminoides 'Lingdi Zhenzhi’

  • 摘要: 为筛选栀子(Gardenia jasminoides)新品种'灵地珍栀’水培扦插繁殖的最优植物生长调节剂组合,提高生根效率与根系发育质量。以'灵地珍栀’半木质化枝条为研究材料,采用3因素3水平的L9(33)正交试验(因素A:IAA、IBA、NAA;因素B:200、300、500 mg·L-1;因素C:浸泡时间2、4、6 h),测定扦插30 d后的生根率、根长、根数及根系效果指数(REI),结合极差分析与方差分析确定最优处理组合。结果表明,调节剂种类(RA=34.81)是影响生根率的主导因子(IBA>IAA>NAA),其次为浓度(RB=18.89)与时间(RC=4.07)。平均根数最多的为IBA 300 mg·L-1浸泡6 h,(1.94±0.54)条;根长最长的为IBA 500 mg·L-1浸泡2 h,(2.12±0.72)cm;而根系效果指数(REI)最高值(1.85±0.66)为IBA 300 mg·L-1浸泡6 h,综合反映根系数量与质量的协同提升。综合生根率、根数、根长及REI等4项指标,采用隶属函数分析结果表明,最佳处理为IBA 200 mg·L-1浸泡4 h,其生根率62.22%、REI 1.42,显著优于其它组合,是促进'灵地珍栀’水培扦插生根的最优植物生长调节剂组合,可平衡生根效率与根系质量。

     

    Abstract: To screen the optimal combination of plant growth regulators for hydroponic cuttings propagation of the new G.jasminoides cultivar 'Lingdi Zhenzhi’,optimizing rooting efficiency and root system development quality. Using semi-lignified branches of 'Lingdi Zhenzhi’ as material,a three-factor,three-level L9(33)orthogonal experiment was conducted(Factor A:IAA,IBA,NAA;Factor B:200,300,500 mg·L-1;Factor C:soaking time 2,4,6 h). Root rate,root length,root number,and root efficiency index(REI)were measured 30 days after cutting. The optimal treatment combination was determined through range analysis and analysis of variance. Results indicated that regulator type(RA=34.81)was the dominant factor influencing rooting rate(IBA > IBA > NAA),followed by concentration(RB=18.89)and duration(RC=4.07). The highest average root number was achieved with IBA 300 mg·L-1 soaked for 6 h(1.94±0.54 roots);The longest root length was achieved with IBA 500 mg·L-1 for 2 h immersion(2.12 ± 0.72 cm). The highest root effectiveness index(REI)of(1.85 ± 0.66)was obtained with IBA 300 mg·L-1 for 6 h immersion,reflecting synergistic enhancement in both root quantity and quality. Based on four indicators-total rooting rate,root number,root length,and REI—membership function analysis identified the optimal treatment as IBA 200 mg·L-1 soaked for 4 h. yielding a rooting rate of 62.22% and an REI of 1.42. This combination significantly outperformed others,establishing it as the optimal plant growth regulator regimen for promoting rooting in hydroponic cuttings of 'Lingdizhenzhi’. This formulation achieves a balanced improvement in both rooting efficiency and root system quality.

     

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