Abstract: Prunus campanulata possesses extremely high ornamental value.However,its large-scale propagation and genetic improvement are severely restricted by the long cycle and low efficiency of traditional breeding methods.To establish an efficient somatic embryogenesis and plant regeneration system for this species,this study used immature zygotic embryos of P.campanulata as explants to systematically investigate the effects of sterilization time,inoculation method,basal medium type,and plant growth regulator combinations on somatic embryogenesis.The results showed that treatment with 1% NaClO for 15 min controlled the explant contamination rate at 10.63% while maintaining high induction activity.Inoculating cotyledons with attached embryonic axes in an inverted position significantly improved somatic embryo induction efficiency.The direct somatic embryo induction rate reached 25.93%,which was 18.52-fold higher than that of horizontally placed cotyledons with excised hypocotyls.Furthermore,the high-salt MS basal medium was significantly superior to the B5 medium in callus induction and embryogenic maintenance.The optimal medium for embryogenic callus induction was MS+2,4-D 2.0 mg·L^-1+6-BA 0.5 mg·L^-1,whereas the optimal medium for somatic embryo differentiation was MS+2,4-D 1.0 mg·L^-1+6-BA 0.5 mg·L^-1,with the latter being more conducive to the formation and development of globular embryos.The germination rate of mature somatic embryos reached up to 65%,and the regenerated plants exhibited a high survival rate and robust growth after acclimatization and transplanting.This study successfully established a regeneration system for P.campanulata primarily based on indirect somatic embryogenesis.This provides an important technical reference for germplasm conservation,genetic engineering breeding,and large-scale propagation of this species.